NOTCH2 anticorps (N-Term)

N° du produit ABIN2470258

Cet anticorps Lapin Polyclonal détecte spécifiquement NOTCH2 dans WB, ELISA, IHC et IF. Il présente une réactivité envers Humain, Chien et Chimpanzé.

Aperçu rapide pour NOTCH2 anticorps (N-Term) (ABIN2470258)

Antigène: NOTCH2 (Notch 2 (NOTCH2))

Reactivité: Humain, Chien, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOTCH2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-NOTCH2 anticorps

Épitope: N-Term

Purification: Sterile Filtration

Immunogène: NOTCH2 antibody was raised against amino acid residues 2396 - 2409 of NOTCH2. A residue of cysteine was added to the amino terminal end to facilitate coupling (Human).

Information d'application

Indications d'application: NOTCH2 antibody can be used in Western Blot starting at 0.5 - 4 μg/mL, and immunohistochemistry starting at 5 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies avoid freeze/thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: Store NOTCH2 antibody at 4 °C or -20 °C.

Détails sur NOTCH2

Antigène: NOTCH2 (Notch 2 (NOTCH2))

Autre désignation: NOTCH2

Sujet: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

ID gène: 4853

UniProt: Q04721

Pathways: Signalisation Notch, Stem Cell Maintenance